Myc shrna
Web25 feb. 2024 · MYC drives the tumorigenesis of human cancers, including prostate cancer (PrCa), thus deubiquitinase (DUB) that maintains high level of c-Myc oncoprotein is a rational therapeutic target. Several ... WebCont, control. (f) Effects of c-Myc shRNA on the Axl promoter activity by ADI. The cells were transfected with c-Myc shRNA and its control scramble shRNA for 48 h. Cells were treated with ADI for 6 h.
Myc shrna
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Web1 jul. 2011 · In this study, we used a lentivirus vector-based RNAi approach which expresses short hairpin RNA (shRNA) to knockdown MYCN in neuroblastoma cell lines IMR-32 and … Web1 jul. 2024 · EMS connects c-Myc to cell cycle control and tumorigenesis. ( A) A549 cells were infected with lentiviruses expressing control shRNA (shctrl), c-Myc shRNA (shc-Myc), or both c-Myc shRNA and EMS. Forty-eight hours later, cells were incubated with actinomycin D (2 μg/mL) for the indicated periods of time.
Web12 jan. 2012 · c-MYC is a protooncogene with a deregulated expression that is associated with the development of tumors in mice and humans; for this reason, its role in tumor cell biology has been investigated extensively. 17 This transcription factor is also involved in several processes in nontransformed cells, including cell growth and apoptosis/survival. … Weband c-Myc shRNA (m) Lentiviral Particles: sc-29227-V. c-Myc (N-262) X TransCruz antibody is recommended for Gel Supershift and ChIP applications. Molecular Weight of c-Myc: 67 kDa. STORAGE Store at 4° C, **DO NOT FREEZE**. Stabl efor one year from the date of shipment. Non-hazardous.
WebCatalog number: SH821514. Description: Four pre-designed shRNA constructs targeting v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) and one scrambled … WebUpon transient transfection with plasmid-encoding shRNA, it was found that expression of c-Myc decreased in shRNA-transfected cells, and the downregulation of c-Myc inhibited cell growth and induced apoptosis in Colo 320 cells. c-Myc downregulation also increased cell population in the G0-G1 phase. In conclusion, our findings demonstrate that ...
WebU2OS MYC/ER cells were infected with lentiviral POLRMT shRNA or Luciferase (Luc) shRNA as a control. Five days post-infection cells were seeded at equal densities and MYC activity was induced via ...
Web12 jun. 2024 · The sEVs of Myc-manipulated SCLC cells were isolated using total exosome isolation reagents (from cells) (Life Technologies, USA). The sEVs were harvested from H82 cont-shRNA, H82 Myc-shRNA, H209 Mock, and H209MYC cells cultured in RPMI-1640 medium supplemented with 10% exosome-depleted FBS in 25 cm 2 flasks and 6-well … breast tissue po polskuWebshRNA 质粒产品一般是由三到五种慢病毒载体质粒混合组成，每个质粒都含有靶基因特异编码的 19-25 nt 的 shRNA（另加发卡结构） 用于敲除基因表达; shRNA 序列 与 Myc … take out keene nhWeb14 jun. 2024 · This study explores the effects of C-myc gene silencing on cell proliferation, apoptosis and cytokine expression in interleukin (IL)-1β-induced rat chondrocytes. Primary chondrocytes were obtained from 40 Sprague-Dawley rats. For in vitro C-myc3-shRNA transfection, chondrocytes were assigned to a blank 1, model 1, IL-1β + C-myc3-shRNA, … breast size kaise kam kare medicinebreast u2Web11 apr. 2024 · STAT3 inhibition attenuates MYC expression by affecting recruitment of histone acetyltransferase p300, thereby reducing enrichment of H3K27 acetylation in the MYC promoter. Concomitantly, it also decreases the occupancy of the bromodomain containing ... using inducible STAT3 shRNA and a clinically relevant STAT3 inhibitor … take out geneva ilWeb12 okt. 2024 · MYC proto-oncogene family including c-myc and n-myc (MYCN) are critical for normal cell development and tumorigenesis. Overexpression of c-myc causes acute … takeout club ksuWebFigure 1 Knockdown of PCGF1 by lentivirus-mediated short hairpin RNA (shRNA)-induced cell cycle arrested at G0/G1 phase and attenuation of proliferation in U87 cells.Notes: (A) Expression analysis of PCGF1 mRNA by RT-qPCR analysis.(B) Expression of PCGF1 proteins in infected cells was analyzed by Western blot.(C) Cell cycle distribution of U87 … breast u5