Peasy®-blunt cloning vector
WebAug 9, 2024 · The pJET1.2/blunt cloning vector is a component of the CloneJET PCR Cloning Kit (Catalogue number: K1231), and it is not sold individually. It contains an eco47IR gene that encodes for a restriction enzyme Eco 47I that is harmful to the common laboratory E. coli strains used in molecular cloning. WebThe pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid Ligation Buffer …
Peasy®-blunt cloning vector
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Webligated to the pEasy-Blunt vector, and the resulting plasmids were then digested with restriction enzymes indicated in Table S2.The gel purified fragments were then ligated to the pETDuet-1 vector that had been digested with the same restriction enzymes to construct the plasmids for the expression of Aldo, DHAD, ALS and ALDC. WebSep 8, 2024 · The PCR product was inserted into the pEASY-Blunt Simple Cloning vector using ClonExpress ... Since the pMDC123 vector has a Bar resistance gene that makes it resistant to the herbicide glufosinate, 1.0 mg·L −1 of glufosinate-ammonium was used to pick out the transformants. The shoots grown back were planted in 1/2 Murashige and …
Webelectrophoresis and ligated it into pEASY®-Blunt Zero Cloning Vector (TransGen Biotech Beijing Co., LTD). Then, the recombinant plasmids transformed into the resistant competent cell. The positive clones were screened and cultured in LB (10 mL) with ampicillin for 5 h. The recombinant plasmids were obtained by plasmid
WebThe Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector contains the lethal E. coliccd B gene fused to the C-terminus of Lac Zα (Bernard et al., 1994). WebApr 13, 2024 · For probe labelling, specific 500 bp sequences of target genes were amplified from the complementary DNA (cDNA) of Wm82 with primers (Supplementary Data 9) and cloned to pEASY-Blunt cloning vector ...
WebpEASY® - T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 minutes fast ligation of Taq-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection.
Web本发明公开了一种拟南芥长链非编码RNA‑DANA1及其在植物干旱耐受性的应用，涉及植物的基因工程领域。其中，所述的DANA1核苷酸序列如SEQ ID NO.1所示。本发明通过克隆拟南芥长链非编码RNA‑DANA1的全长转录本，并构建过表达载体获得DANA1过表达转基因植株，过表达DANA1后植物表现出失水速率减慢，对 ... shires funeral directors sandyWebJan 5, 2024 · Forward and reverse oligonucleotides for sgRNA cloning into the targeting vector. 2. Restriction enzymes: BsaI and PvuI. 3. Easytaq DNA polymerase for E. coli colony PCR and for identifying plants with gene replacement. 4. FastPfu DNA polymerase for generating the donor sequences. 5. pEASY-Blunt cloning vector for donor construction. 6. quiz bee about united nationsWebIntroduction Product Overview Description of the system The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of nonrecombinants. The - pCR®-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994).Ligation of a blunt PCR fragment disrupts … shires funeral directors baldockWebMay 28, 2024 · A 2798 bp (− 2518 bp ~ + 280 bp; translation initiation site "ATG" was numbered as + 1) region of Ppd-D1b promoter was amplified from the wheat cultivar Chinese spring genomic DNA and cloned into pEASY-Blunt Cloning vectors, which was confirmed by sequencing. shires funeral baldockWebSep 1, 2024 · Removal of the MCS fragment from the pJET1.2M plasmid produces the pJET1.2/blunt cloning vector ready for gene cloning. The self-made pJET1.2/blunt cloning vector exhibited a... quiz bee about philippine historyWebThe Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room … quiz bee englishWebSep 2, 2024 · The pEASY-Blunt cloning vector is an intermediate vector. MCS, multiple cloning site. MCS, multiple cloning site. The plasmid pBHt2-OsPEF1α-GFP contains two gene expression cassettes: the hygromycin B phosphotransferase enzyme ( HPH ) gene cassette with the Aspergillus nidulans trpC promoter and CaMV 35S terminator and the … quiz bee biology