WebFor PCR amplification, AccuStart II PCR ToughMix measured using a NanoDrop and then pooled in the following manner: (Quantabio) reaction mix was used, which improves reproducibility, 9 ng from Cyt b, 8.7 ng from 12S, and 7.3 ng from 16S, to obtain a reduces the risk of contamination, and performs well in the total of 25 ng of DNA. WebOct 6, 2024 · rRT-PCR for CDC2024 was performed using the qScript™ XLT One-Step RT-qPCR ToughMix® (Quanta Biosciences; catalog #95132-500 or 95132-100). The reaction components and thermocycling conditions are provided in detail in Tables S1 and S2. Briefly, the 20 µl reaction mixture contained 10 µl of ToughMix enzyme reagent,
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